Skip to main content
. 2014 Apr 15;9(4):e95329. doi: 10.1371/journal.pone.0095329

Figure 1. Conversion of ESCs into INTPSCs in a modified EpiSC culture condition containing CHIR99021.

Figure 1

(A) Proliferation of ESCs in the presence of LIF/2i, F, FA, and FAC. (B) Proportions of Oct3/4-GFP–positive ESCs in the presence of LIF/2i, F, FA, and FAC. (C) Phase-contrast images of ESCs, ESC-INTPSCs, and EpiSCs. Scale bar, 50 µm. (D) Alkaline-phosphatase staining of ESCs and ESC-INTPSCs. Scale bar, 500 µm. (E and G) Immunostaining of ESCs and ESC-INTPSCs. Scale bar, 500 µm. (F) Percentage of OCT3/4-positive or -negative cells in GOF18 Oct3/4-GFP–positive or –negative ESCs, ESC-INTPSCs, and EpiSCs. (H) Immunostaining for H3K27me3 in E6.0 epiblasts, ESCs, and ESC-INTPSCs. Scale bar, 10 µm.