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. 2014 Apr 15;9(4):e95396. doi: 10.1371/journal.pone.0095396

Figure 2. The 5′ leader of the CLV1 mRNA renders translation dependent on eIF3h.

Figure 2

(A) The 5′ leader of CLV1 harbors multiple uORFs. The boxes stand for uORFs that are in the –1 frame (green), or in the +1 frame (blue) with the main ORF. The cDNA sequence corresponds to the longest known gene model: CLV1 (At1g75820.1). (B) Schematic view of the mRNAs for protoplast transformation. mRNAs were prepared by in vitro transcription with SP6 RNA polymerase. An equal amount of internal control (Spacer-LUC+) mRNA was added to the 5′ leader-RLUC mRNA to be tested as an internal control for transformation efficiency. (C) Translational efficiency on the CLV1 and WUS 5′ leader is expressed as the mean RLUC/FLUC ratio with standard errors from three replicate transformations.