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. 2014 Apr 15;9(4):e95396. doi: 10.1371/journal.pone.0095396

Figure 4. Removal of uORFs from the CLV1 5′ leader reduces its eIF3h dependence.

Figure 4

(A) Transient dual luciferase assays were performed in protoplasts as in Figure 2 but data are presented as the relative expression in the eif3h mutant as compared to wild type with standard deviations. Asterisks represent up to five uAUGs and open boxes represent uORFs. (B) Stable transgenic plants harboring the dual-luciferase construct illustrated at the top. The CLV1 native leader or uORF-less leader is linked to FLUC, and the RLUC ORF driven by the crTMV-element serves as a reference. Translational efficiency of the 5′ leader was established via dual luciferase assays in 10-day-old seedlings from three independent transgenic lines. Statistical significance was determined by t-test (***for p-value <0.001 and **for P-value<0.05). x0.05: FLUC activities of one particularly highly expressing line were multiplied by 0.05 for convenience of display.