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. 2014 Apr 15;9(4):e95396. doi: 10.1371/journal.pone.0095396

Table 2. Polysome loading state of selected mRNAs in eif3h mutant versus wild type.

Gene AGI TL eif3h/WT uORFs
CLV1 At1g75820 −0.89 4
AS1 At2g37630 −1.44 3
TOADSTOOL At3g02130 −1.65 NA
CORYNE At5g13290 −0.36 4
CLV2 At1g65380 −0.46 0
CORONA At1g52150 −0.59 8
SPLAYED At2g28290 −0.20 2
KNAT1/BP At4g08150 −0.40 2
ARF4 At5g60450 −0.59 3
ARF5 At1g19850 −0.48 6
ARF6 At1g30330 −0.20 6
ARF7 At5g20730 −0.61 4
ARF11 At2g46530 −0.47 6
ARF18 At3g61830 −0.76 2
HY5 At5g11260 +0.17 1
ATBZIP11 At4g34590 −0.65 4
TIR1 At3g62980 +0.18 1
AUX1 At2g38120 +0.31 1

Note: TL eif3h/WT. Translation state (TL) is the ratio of mRNA in polysomal and non-polysomal RNA fractions; it is log-transformed and unitless. When compared between eif3h mutant and wild-type seedlings, negative numbers indicate that the mRNA is less polysomal, i.e. undertranslated in the eif3h mutant. The values are means from duplicate polysome microarrays [13]. TOADSTOOL and the heterodimer of CLV2 and CORYNE are receptor like kinases that can function as CLV3 receptors in parallel to CLV1. Data for ARFs, TIR1 and AUX1 are included for the purpose of calibration, given that ARFs are also undertranslated in eif3h while TIR1 and AUX1 are not [16]. The median value from 8832 genes was −0.10±0.21. TOADSTOOL and AS1 and CLV1 rank 19th and 55th and 495th, respectively, among cytosolic mRNAs. uORF numbers are from TAIR10. NA, not available.