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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Nutr Biochem. 2014 Feb 4;25(5):515–525. doi: 10.1016/j.jnutbio.2013.12.011

Figure 3. Effect of DHA on HO-1 gene transcription.

Figure 3

A. A2780 cells were transfected with the PGL3/4.5-HO-1 promoter reporter construct and treated with DHA at the indicated concentrations for 1, 4 or 21 hours. Cell lysates were prepared, and luciferase activity was assayed. Data are expressed as percentages of the value detected in untreated control cells (bars, SEM; n=3). **, P<0.01, *, P<0.05, compared to untreated control cells, using one-way ANOVA followed by Dunnett’s analysis. B. A2780 cells were transfected with the HO-1-3′-UTR or the GAPDH-3′-UTR reporter construct and treated with 100 μM DHA for 1, 4 or 21 hours. Cell lysates were prepared, and luciferase activity was assayed. Data are expressed as percentages of the value detected in untreated control cells (bars, SEM; n=3).