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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Nutr Biochem. 2014 Feb 4;25(5):515–525. doi: 10.1016/j.jnutbio.2013.12.011

Figure 8. Effects of proteasome inhibitors on the DHA-induced increase in HO-1 promoter activity.

Figure 8

A. A2780 cells were transfected with the PGL3/4.5-HO-1 promoter reporter construct. Forty-eight hours post-transfection the cells were pre-treated with 10 μM MG132 or 1 μM bortezomib for 15 minutes followed by treatment with 100 μM DHA for 21 hours. Cell lysates were prepared and luciferase activity was assayed. Data are expressed as percentages of the value detected in untreated control cells (bar, SEM, n=3). **, P<0.01, compared to untreated control cells, using one-way ANOVA followed by Dunnett’s analysis. B. A2780 cells were treated with 10 μM MG132, 100 μM DHA, or pre-treated with 10 μM MG132 for 15 minutes followed by 100 μM DHA for 21 hours. Cell lysates were prepared and Western blot was performed using antibodies against Bach1 and β-actin. Shown are representative images of 3 separate experiments. The bands were quantified, normalized to β-actin and is expressed as percentages relative to the expression level in untreated controls. *, P<0.05, **, p<0.01, using one-way ANOVA followed by Dunnett’s analysis.