Skip to main content
. 2014 Apr 15;9(4):e95197. doi: 10.1371/journal.pone.0095197

Figure 3. GFP-LC3 and SV5-LC3 fusion constructs in RV-infected cells.

Figure 3

Confocal immunofluorescence of MA104 cells transiently over-expressing the fusion construct GFP-LC3 (A) or SV5-LC3 (B). Cells were untreated, RV-infected (OSU strain; MOI, 5; 13 hpi) or treated from 1 hpi to 13 hpi with RAP (0.1 µM), CQ (50 µM), or torin 1 (0.25 µM), as indicated. Viroplasms were visualized with an anti-NSP5 antibody (red) and the fusion constructs with the GFP fluorescence in A and with an anti-SV5 antibody in B (green). Single optical sections are shown. Scale bar, 10 µm. Images are representative of three independent experiments in which at least 150 cells per each experimental condition were analyzed.