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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Brain Res Bull. 2013 Oct 15;0:39–48. doi: 10.1016/j.brainresbull.2013.10.004

Figure 2.

Figure 2

Chronic disinhibition reduces levels of phosphorylated CREB (pCREB) in CA1 hippocampal pyramidal cells. (A) Low magnification confocal image of a hippocampal slice culture showing typical pattern of immunohistochemical staining for pCREB. (B and C) Comparison of pCREB staining in hippocampal area CA1 in a control slice culture (B) and a slice treated for 4 days with bicuculline (C). Notice the dramatic reduction in expression levels of pCREB. Also shown are the subpopulation of CA1 pyramidal cells that express YFP in YFP-H mice. (D-F) The nuclear localization of pCREB is YFP-positive CA1 pyramidal cells is clearly illustrated in merged images from a control slice. (G-I) Following 4 days of bicuculline treatment pCREB immunoreactivity in CA1 pyramidal cells remains nuclear but is greatly reduced in intensity. The majority of pCREB positive –YFP negative cells are CA1 pyramidal cells that do not express YFP. Arrow heads denote sites of co-localization. Scale bars: A = 500 μm. B and C = 100 μm. D – I = 20 μm.