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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Cell. 2013 Dec 19;156(0):158–169. doi: 10.1016/j.cell.2013.11.031

Figure 5. The Natural MF-Pre5 and MI-Sry1 Proteins Contain Met-Based N-Degrons.

Figure 5

(A) CHX chases with MF-Pre5ha in WT (lanes 1–3), ubr1Δ (lanes 4–6), naa30Δ (lanes 7–9), and naa30Δ ubr1Δ cells (lanes 10–12).

(B) Growth rates of WT, naa30Δ and naa30Δ ubr1Δ S. cerevisiae strains, including WT and naa30Δ strains that overexpressed the MF-Pre5 proteasomal subunit. Standard errors (of triplicate measurements) are shown as well.

(C) CHX chases with MI-Sry1ha (lanes 1–3) and MK-Sry1ha (lanes 4–6) in naa30Δ cells.

(D) CHX chases with endogenously expressed MI-Sry1ha in WT (lanes 1–3), ubr1Δ (lanes 4–6), naa30Δ (lanes 7–9), and naa30Δ ubr1Δ cells (lanes 10–12). See also Figures S1, S2, and S4.