Fig. 4.

Biotic stress assay of T₁ progenies of glucanase transgenic lines and untransformed control in root dip and soil mix fungal resistance assays against infection by F. oxysporum and V. dahliae. a In vivo root dip assay using V. dahliae. b In vivo root dip assay using F. oxysporum. c In vivo soil mix assay using V. dahliae. d In vivo soil mix assay using F. oxysporum. Wt indicates untransformed control. Glu2, Glu12, Glu14, and Glu17 indicate transgenic lines. Data were recorded after 1 month of inoculation. 6–8 T₁ progeny seedlings per transgenic line tested for all the experiments. The experiments were repeated thrice with 6–8 T₁ progeny seedlings per transgenic lines tested