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(A) Human endothelial colony-forming cells (ECFCs) grow in culture as a cell monolayer and disclose a cobblestone appearance. Cells form tight junctional complexes, shown by Z0-1 staining in green. Nuclei labeled in blue with DAPI. (B) Human endothelial colony-forming cells (ECFCs) (labeled in red) form tube-like structures with retinal microvascular endothelial cells (labeled in green) in a 3D Matrigel in vitro model.
