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. 2013 Dec 5;1:29. doi: 10.3389/fchem.2013.00029

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Copyright © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal Laser Scanning Microscopy (CLSM) in vivo images of internalized AgNPs (stabilized with compound 3) in HepG2 cells after 24 h of treatment at 100 μg/ml. Confocal images obtained of a single channels of nanoparticle fluorescence (green fluorescence), nanoparticle reflection brightness (gray), nuclear stain (DAPI), and membrane stain (Cell Mask) are shown. Merge images of all individual channels (merge), three dimensional reconstruction (3D) and Z projection (Z) allows to visualize the AgNPs particles into HepG2 treated cells. White arrows indicate the location of internalized AgNPs.