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. 2014 Apr 16;34(16):5717–5731. doi: 10.1523/JNEUROSCI.4256-13.2014

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Copyright © 2014 the authors 2014 Yeh et al.

This article is freely available online through the J Neurosci Author Open Choice option.

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Figure 5. — Reduced proliferation in Robo1^+/+, but not in Robo1^−/− dissociated cortical cell cultures following Slit1/Slit2 treatment. A, B, Histograms show percentage of BrdU⁺ E15.5 rat apical (Pax6⁺; A) and basal (Tbr2⁺; B) progenitor cells following treatment with either: Control, Slit1, Slit2, Slit1/Slit2, Robo1-Fc, or Nrp1-Fc overnight. Treatment with Slit1/Slit2 and Robo1-Fc caused a significant decrease in proliferation of both progenitor pools. C, D, Dissociated cortical cell cultures prepared from E13.5 Robo1^+/+ and Robo1^−/− mice were incubated overnight in the presence or absence of Slit1/Slit2, and the percentages of proliferating apical (C) and basal (D) progenitors were assessed following a 2 h BrdU pulse. Wild-type cultures showed a significant decrease in proliferation following Slit1/Slit2 treatment. Robo1^−/− dissociated cortical cell cultures were unaffected by Slit treatment, but showed increased proliferation compared with wild-type cultures (E); *p < 0.05, **p < 0.004, ***p < 0.0004.