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. 2011 Mar 29;4(3):516–526. doi: 10.1093/mp/ssr016

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© The Author 2011. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

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Figure 1. — The pht4;1-1 Mutant Suppresses acd6-1-Conferred Phenotypes.

(A) Picture of 25-day-old plants.

(B) Northern blot analysis of PR1 expression. Total RNA was isolated from 25-day-old uninfected plants. rRNA was used as a loading control.

(C) SA quantification. Uninfected 25-day-old plants were harvested for SA extraction followed by HPLC analysis.

(D) Bacterial growth assay. 25-day-old plants were infected with Pseudomonas syringae pv. maculicola strain DG3 (PmaDG3) (OD₆₀₀ = 0.0001) and leaf discs were collected for bacterial growth assay 3 d after infection. CFU, colony forming unit.

Different letters in (C) and (D) indicate significant difference among samples (P < 0.05; n = 3 in (C) and n = 6 in (D)). These experiments were repeated two times, with similar results.