Figure 4.

Transgenic Plants Expressing PHT4;1-1 or PHT4;1 Are More Susceptible to PmaDG3 Infection.

A genomic fragment containing the PHT4;1-1 region or the full-length PHT4;1 gene was PCR amplified and cloned into the binary vector pGreenII 0029 (Hellens et al., 2000) for plant transformation. The PHT4;1 promoter (1329 bp) was used to drive the expression of these two constructs. 25-day-old plants were infected with PmaDG3 (OD₆₀₀ = 0.0001) and leaf discs were collected for bacterial growth assay 3 d after infection. Asterisks indicate significant difference between Col-0 and the transgenic plants (P < 0.05; n = 6). These experiments were repeated two times, with similar results.