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. Author manuscript; available in PMC: 2014 Apr 16.
Published in final edited form as: Mol Microbiol. 2012 Aug 24;86(2):457–471. doi: 10.1111/j.1365-2958.2012.08205.x

Fig. 7. Epistasis analysis of the ΔftsH mutant and ΔfloT ΔyqfA double mutant to restore biofilm formation.

Fig. 7

A. Pellicle formation of the indicated strains of B. subtilis. Pictures show a top view of the pellicles formed on the surface of MSgg incubated in 24-well plates at 30°C for 24 h. Positive control is represented by the wild-type strain (WT) and negative controls are represented by the ΔfloT ΔyqfA double mutant (strain JS163) and the ΔftsH mutant (DL1308).

B. Viable spore counts comparing WT, ΔfloT ΔyqfA and ΔftsH strains when complemented with the sad67 variant (strains DL1148, DL1375 and DL1363 respectively) or with a deletion of the FtsH-regulated phosphatases (rapA, rapB, rapE and spo0E) (strains DL1430, DL1554 and DL1375 respectively). sad67 was expressed under the control of an IPTG-inducible promoter with 1 μM IPTG. Cultures were grown in shaking MSgg at 30°C for 48 h. Number of spores was correlated to the optical density of the cultures. Error bars indicate standard error of the means.