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. Author manuscript; available in PMC: 2014 Apr 16.
Published in final edited form as: Mol Microbiol. 2012 Aug 24;86(2):457–471. doi: 10.1111/j.1365-2958.2012.08205.x

Fig. 8. SpoVM protein inhibits differentiation of matrix producers and biofilm formation.

Fig. 8

A. Pellicle formation of B. subtilis wild-type (WT) or rapA, rapB, rapE and spo0E mutant in the presence or absence (control) of the protein SpoVM (50 nM).

B. Flow cytometry monitoring the expression of the reporter PtapA-yfp (YFP fluorescence on the y-axis) and Phag-cfp (CFP fluorescence on the x-axis) from B. subtilis cells grown on the pellicle formation assay. Fluorescence for both CFP and YFP in a strain harbouring both reporters, PtapA-yfp and Phag-cfp which correspond to the matrix producers and motile cells, framed in yellow and blue respectively. Non-treated (top panel) or treated with the SpoVM protein 50 nM (bottom panel).