Table 3.

Primers used for sequence analysis.

Gene	Region	Primer sequences (5′ 3′)- Forward	Primer sequences (5′ 3′)- Reverse	Amplicon size (bp)	Annealing Temperature
MST4	Exon 1	GCGCCAGAAAGGTAGACTGA	ACACCAGGAAACACCTCCAG	371	58°C
	Exon 2	TGGGGAGTCAGAGGAGTTTG	GATTCTGCCTGGAAGCCATA	486	54°C
	Exon 3	GCTCCCACAAATAAATGAGAACA	AATGCCAGCAACATGTTCAA	381	53°C
	Exon 4	GGAACCTCAGAATTTGAAGCCTA	TTGGCAGCTAGGAGGAAAAA	483	54°C
	Exon 5	TTGGAATGGAGTTTCAAGGTG	AACCAGGTTCCTGGATGGAT	420	52°C
	Exon 6	TTGGAATGGAGTTTCAAGGTG	CCCTACTGCAGGAAAAGCTG	372	52°C
	Exon 7	TTTTCCTACAGCATTTTAGTTGTTC	CAAGCACACTGGAGCATAGC	377	51°C
	Exon 8	AGTATTCGCCTGAAGCTGAGA	AGTGAGGCAGACAGGCATTT	367	55°C
	Exon 9 and 10	TTTTGTTTTCAGTAGGGGATTCA	TTGGAATGGTTGAATCAAAGG	482	51°C
	Exon 11 and UTR1	TGTGAAATCAGATGCAGAAGG	CATCCTCGTAACTTAAGAAATAGGG	497	53°C
	UTR2	TTTGTGATGGCGTTTATCATTT	CCAACTTGGTAGAAGAGTCAATACTTT	400	51°C
	UTR3	CAGGTCTTTCAAAGTCATTCTCAA	GCTCTCCAAATCCAAGCAGA	483	53°C
	UTR4	GGCAATTTTACCCTTATTTCACA	TTTGTGTGGACAGAAATCATCC	389	51°C
	UTR5	TTTGCCATGGTAATAAATTGTCC	GGATGTAATGGCCACTTTGAC	469	51°C
	UTR6	TGGGGCTGTATTTCAGTAGTTG	TTGGGATGCAATTGTGTTGT	498	53°C
	UTR7	TTGTGGTGTTGTATGCCAAGA	TGAAAAGTATCTTCTGGTTTCCA	498	52°C
FGF13	Exon 1	CAAACGCAGGCTAAGGAGAC	TGTTACGGAGGCAAGAAAGC	484	56 °C
	Exon 2	TTGTTTTCTTTCTTCCAGACACC	TTCAGGATTTAGGAAAGCGTATTT	353	53 °C
	Exon 3	GCAGTCCTGCTGGAAACATT	AAAATGCTTGCTTCAAATAACTG	380	53 °C
	Exon 4	CCTAAAAGGCAATACCACATGA	ACCACCTGTACCCCCAAAAC	370	52 °C
	Exon 5	TCATTTAGGTGTGTGGAATAGAACA	TGCTTGGCATTCTTATGCAC	456	54 °C
	UTR1	GGTGTGTGGAATAGAACACCTACA	GAACTCTGCCTGTTTGTTTGG	363	55 °C
	UTR2	CAAATCCATGAGCCACAATG	ATTTTCTTGGTGGGAGAGCA	373	53 °C
	UTR3	TCTGAGAGAAGGACTGCCAAA	CTTGAGCCTGAGAAGCCAAT	390	55 °C
	UTR4	TGTTGTTTTCTTGGCTTGATG	TGCCAAATACAATGACAGGAAG	500	59 °C
	UTR5	CCTCAAGTGTAGATTTCTCTTAATTCC	TGTCCACTTTTGGAACACAGAT	386	54 °C
	UTR6	CTGGTTTTCCCTCAACAACA	CCTTTGTACCAATCTGAAGTGC	500	54 °C