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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Dev Biol. 2014 Feb 21;389(2):124–136. doi: 10.1016/j.ydbio.2014.01.025

Fig. 6. Ablation of Lhx1 leads to decreased cell proliferation in the Müllerian duct epithelium.

Fig. 6

A, B, Immunofluorescence for phospho-histone H3 (pH3, red) at E12.5 in the trunk region demonstrates that there is a large amount of cell proliferation in the control Müllerian duct epithelium, whereas in Lhx1 cKO embryos there is much less proliferation in the Müllerian duct epithelium. C, Statistical analysis shows a significant difference in mitotic rate of the Müllerian duct between control (36.4 ± 2.4%, mean ± SEM) and Lhx1 cKO (15.5 ± 0.5%) embryos. T-test: p ≤ 0.01. Error bars represent SEM. D, E, Immunofluorescence for pH3 (red) at E12.5 shows a large amount of cell proliferation in the control Müllerian duct epithelium but much less proliferation is detected in the Müllerian duct epithelium tip region of the Lhx1 cKO embryos. Green, immunofluorescent labeling of pan-cytokeratin; blue, DAPI. F, Statistical analysis shows the difference in mitotic rate in the Müllerian tip between control (36.0 ± 4.2%) and Lhx1 cKO (22.7 ± 2.1%) embryos is also significant. T-test: p < 0.05. Error bars SEM. Scale bars represent 50μm in A, B, D and E and 5 μm in insets in A and B.