Fig. 4.

Effect of extracelluar Ca²⁺-free conditions on naringenin-induced increases in [Ca²⁺]_i. Cells were loaded with a calcium-indicator dye in the absence or presence of 1.25 mM Ca²⁺ for 60 min. Values are expressed as mean ± SEM from three separate experiments performed in triplicate (n = 9). ^***P < 0.001 vs. [Ca²⁺]_i increases induced by naringenin in 1.25 mM Ca²⁺.