Fig. 4.

Effect of peanut sprout extracts on leptin protein expression in adipose tissue of rats. Frozen adipose tissue was homogenized in lysis buffer. Protein concentration was determined by using a Bio-Rad method. Equal amounts of proteins (30 µg) were resolved by SDS-PAGE, transferred to the membranes and probed with leptin. Above photographs of chemiliuminiscent detection of the western blots, are shown and under the graph of quantitative analysis which were representatives of three independent experiments. Each bar represents the mean ± SE