Figure 4.

Effect of pretreatment of HDAC inhibitors on the acetylation patterns at the c-fos gene promoter in the nucleus accumbens shell and prefrontal cortex of HAL-treated young and aged mice. Acetylation of H3K27 and H4K12 at the c-fos promoter of the nucleus accumbens shell (a and b) and prefrontal cortex (c and d) from young (gray bars) and aged (black bars) mice was measured as the percentage of input with chromatin immunoprecipitation assay. Data are presented as mean±SEM, n=4–5. *p<0.05, **p<0.01, ***p<0.001.