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Stopped-flow fluorescent analysis of RF1 dissociation from ribosomes promoted by RF3-nucleotide. (A) The dissociation of RF1 from the ribosome in the presence and absence of RF3:GTP as monitored by stopped-flow fluorescence. The rates of RF1 (B) and GAQ-RF1 (C) dissociation from termination complexes with no RF3, RF3:GDP, and RF3:GTP present are displayed.
