Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May;20(5):656–669. doi: 10.1261/rna.042838.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Hadjivassiliou et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 2. — Sad1 does not bind ubiquitin in vitro. (A) A primary sequence alignment of ubiquitin binding and nonubiquitin binding Homo sapiens and S. cerevisiae ZnF-UBP domains. The WRY motif residues that are essential for ubiquitin binding in USP5 are annotated with asterisks; in Sad1, they appear as YRL. The residues that comprise the three zinc fingers found in ZnF-UBPs are denoted A, B, and C; Sad1 only possesses the B site zinc finger. Conservation is indicated by shades of gray, and gaps in the alignment are represented by dashes. (B) We titrated 1 mM of ubiquitin into an ITC receptor cell containing either 50 µM full-length Sad1, Sad1-iUSP, USP5 ZnF-UBP, or buffer at 25°C. The data from the USP5 ZnF-UBP titration with ubiquitin were fit to a single exponential in good agreement with one binding site and a dissociation constant of 2.3 µM. ITC curve-fitting and data analysis were performed with the MicroCal-enabled Origin software.