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. 2014 May;20(5):681–688. doi: 10.1261/rna.043984.113

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© 2014 Kelly et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Neuron-specific expression of ZC3H14-iso1 can rescue viability defects of homozygous dNab2^nullflies. (A) The percentage of adult flies eclosed (relative to expected Mendelian ratios) for each of the indicated genotypes is summarized. Genotypes are as follows: dNab2 null (dNab2^ex3/dNab2^ex3), +dNab2 transgene alone (UAS-dNab2^Flag/+;;dNab2^ex3/dNab2^ex3), +dNab2 expressed in neurons (UAS-dNab2^Flag/elav-GAL4;;dNab2^ex3/dNab2^ex3), +ZC3H14-iso1 transgene alone (UAS-ZC3H14-iso1^Flag/+;;dNab2^ex3/dNab2^ex3), +ZC3H14-iso1 expressed in neurons (UAS-ZC3H14-iso1^Flag/elav-GAL4;;dNab2^ex3/dNab2^ex3), +ZC3H14-iso4 transgene alone (+/+ or +/Y;UAS-ZC3H14-iso4^Flag/+;;dNab2^ex3/dNab2^ex3), +ZC3H14-iso4 expressed in neurons (elav-GAL4/+ or elav-GAL4/Y;UAS-ZC3H14-iso4^Flag/+;dNab2^ex3/dNab2^ex3). (B) Anti-Flag immunoblots to detect pan-neuronal (using elav-GAL4) expression of the UAS-dNab2^Flag, UAS-ZC3H14-iso1^Flag, and UAS-ZC3H14-iso4^Flag transgenes in fly heads. The blot on the right corresponding to ZC3H14 iso4 is a darker exposure (as indicated) due to lower-level expression of ZC3H14 iso4 relative to ZC3H14 iso1. The dNab2 band, which is the same band but shown at the different exposures, for both ZC3H14-iso1 and ZC3H14-iso4, illustrates the relative levels of exposure. Control flies expressing Gal4 only are also shown. The approximate position of molecular weight markers is indicated to the right.