Table 2.

Comparison of C-H amination activities (TTN) of intact E. coli cells expressing P450 and P411 variants. Reaction conditions described in supporting information.

In vivo catalyst	[P450] or [P411] µM	% yield[a] (3)	TTN[b]	% ee[c]
pCWori-empty	0	0	0	nd
P450BM3	6.6	0.5	5.1	nd
P450BM3-T268A	5.8	7.8	26	84
P411BM3	4.3	6.7	29	16
P411BM3-T268A	2.2	30	250	89
P411BM3-CIS	1.4	46	680	60
P411BM3-CIS-T438S	2.7	58	430	87

^[a]TTN = total turnover number

^[b]*(S−R)/(S+R).

nd = not determined.