Table 1.
Attachment of substituents on CPMV. The number of substitutions per virus was measured by UV absorption of fluorescein at 495 nm with the exception of SFB, where the absorption was measured at 350 nm. For simplicity in the table PEG corresponds to PEG500f. For subsequent conjugations, the level of excess substituent added to each reaction is expressed as “fold excess” over the number of attached benzaldehydes.
| Reagents | Control C | SFB | PEG P1 |
PEG P2 |
PEG P3 |
PEG P4 |
F56f F1 |
F56f PEG FP1 |
F56f PEG FP2 |
F56f PEG FP3 |
|---|---|---|---|---|---|---|---|---|---|---|
| fold excess PEG/aldehyde | 10 | 20 | 50 | 100 | 20 | 50 | 100 | |||
| fold excess F56/aldehyde | 100 | 100 | 100 | 100 | ||||||
| fold excess fluorescein/aldehyde | 2 | |||||||||
| Substitutions per CPMV | 75 | 280 | 20 | 42 | 125 | 197 | 133 | 149 | 163 | 188 |