Control male (gray bars, N=12) and LX-exposed male (white bars, N=12) as well as control female (cross-hatched gray bars, N=17) and LX-exposed female mice (cross-hatched white bars, N=18) underwent fear conditioning. On the first day of the protocol, mice were trained to associate a cue (tone) and context (conditioning chamber) with a stressor (foot shock), and fear-related freezing was recorded (A). The following day, the same mice were placed in a different context and cue-based fear was assessed as a test of hippocampal and amygdala function (B).