Skip to main content
. 2014 Mar 31;2014:989434. doi: 10.1155/2014/989434

Figure 2.

Figure 2

Rapamycin improved the expression of FoxP3 and developed the potent suppressive activity in vitro. (a) The proportion of CD4+CD25+FoxP3+ iTreg induced from naïve T cells. (b) CD25 and FoxP3 coexpression in iTreg was assessed by flow cytometry on day 7. (c) Relative CD25 MFI in iTreg cells on day 7 with or without rapamycin. (d) In this representative experiment, the cells were stained for anti-CD8 and the suppressive activity of various primed CD4+ cells subsets on CFSE-labeled CD8+ at various T suppressor to T effector ratios was shown. (e) The mean ± SEM percent suppression of iTreg at the various ratios. The values indicated the mean ± SEM of 3 separate experiments. *P < 0.05.