Figure 2. Deletions of both p53 and mir-34 Promote Prostate Stem Cell Expansion and Sphere-forming Capacity.

(A) Quantitative analysis of distribution of CD49f^hi/Sca-1⁺ stem cells and CD49f^lo/Sca-1⁻ luminal cells from 3-month-old wild-type (WT), mir-34^PE−/−, p53^PE−/−, and p53^PE−/−mir-34^PE−/− mice (n=3). Red and blue frames represent stem cell and luminal cell populations, respectively. (B-D) Frequency (B, D) and size (C) of spheres formed by CD49f^hi/Sca-1⁺ stem cells (B, C) and CD49f^lo/Sca-1⁻ (D) luminal cells from 3-month-old WT, mir-34^PE−/−, p53^PE−/−, and p53^PE−/−mir-34^PE−/− mice (n=3). (E, F) Relative frequency of sphere formation by CD49f^hi/Sca-1⁺ stem cells (E) and CD49f^lo/Sca-1⁻ luminal cells (F) isolated from WT, mir-34^L/L, p53^L/L, and p53^L/Lmir-34^L/L mice followed by Ad-Cre infection (n=3). Spheres counts were normalized to the Ad-blank-infected spheres of each passage. **P<0.01, ***P<0.001. Error bars denote SD.