Fig. 1. Bacterial RNA inhibits direct coilin interaction with WRAP53.

(A–C) GST-pulldown experiments were conducted using GST, GST-coilin (or fragments thereof) and His-T7-tagged WRAP53 partially purified from bacteria. Pulldown reactions were subjected to SDS-PAGE, Western blotting, and probing with the indicated antibodies. RNase A/T1 treated GST proteins were also used (+). The input lane represents 10% of the His-T7-WRAP53 used in each reaction.