Fig. 1. Phenotypes of Ras^V12 transformed Drosophila lines.

(A) Increased size of wing discs and salivary glands in Ras^V12-expressing larvae. Wing disc and salivary gland size was determined in the indicated genotypes using ImageJ. Both Ras^V12 wing discs and salivary glands (grey bar) are significantly larger than parental controls (white bars) (at p = 2.97 E−07 (Bx-Gal) and 0.007 (UAS-Ras^V12), respectively, for the wing discs and 2.53 E−18 and 1.14 E−17, respectively, for the salivary glands). (B) Melanization in flies and larvae expressing Ras^V12. Left: wild-type fly (w¹¹¹⁸). Right: Bx-GAL4;tub-GAL80^ts/UAS-Ras^V12 flies at 18°C, 23°C and pupa at 29°C (note the melanotic spot in the pupa indicated by an arrow). Scale bar: 1 mm.