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. 2014 Mar 27;3(4):289–296. doi: 10.1242/bio.20146585

Fig. 3. Cellular localization of BODIPY-DIF-3R in HeLa cells.

Fig. 3.

(A) Cells were incubated for 0.5 h with BODIPY-DIF-3R (20 µM), Hoechst (0.1 µg/ml), and MitoTrackerG (0.1 µM), washed free of the additives, and observed by using high-magnification fluorescence microscopy. (B) Cells were incubated for 3 days with BODIPY-DIF-3R (20 µM) and then for 0.5 h with Hoechst (0.1 µg/ml) and MitoTrackerG (0.1 µM). Cells were washed free of the additives and observed by using high-magnification fluorescence microscopy. (A,B) Three-dimensional (3D) images were constructed from z-stacked two-dimensional (2D) images, and two representative 2D-projections of the 3D images are shown. Scale bars: 20 µm.