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. 2014 Mar 27;3(4):289–296. doi: 10.1242/bio.20146585

Fig. 5. Cellular localization of BODIPY-DIF-3G and BODIPY-DIF-3R in HeLa cells treated for 3 days with BODIPY-DIF-3G.

Fig. 5.

Cells were incubated for 3 days with BODIPY-DIF-3G (20 µM) and then for 0.5 h with Hoechst (0.1 µg/ml) and MitoTrackerR (0.1 µM) (A) or BODIPY-DIF-3R (20 µM) (B). Cells were washed free of the additives and observed by using high-magnification fluorescence microscopy. Three-dimensional (3D) images were constructed from z-stacked two-dimensional (2D) images, and two representative 2D-projections of the 3D images are shown. BODIPY-DIF-3G and MitoTrackerR co-localized to mitochondria, and the swollen mitochondria were stained with BODIPY-DIF-3G and MitoTrackerR, but not BODIPY-DIF-3R. Scale bars: 20 µm.