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. 2014 Mar 27;3(4):289–296. doi: 10.1242/bio.20146585

Fig. 7. Cellular localization of BODIPY-DIF-3G and BODIPY-DIF-3R in formalin-fixed HeLa cells.

Fig. 7.

Cells were incubated for 3 days without (A) or with (B) CCCP (10 µM) and then incubated for a further 0.5 h with Hoechst (0.1 µg/ml) and MitoTrackerDR (0.2 µM). Cells were washed free of the additives and fixed with 3.7% formalin. The fixed cells were then stained for 0.5 h with BODIPY-DIF-3G (20 µM) and BODIPY-DIF-3R (20 µM), washed free of the additives, and observed by using high-magnification fluorescence microscopy. Merged images (e,f) were constructed with images of cells stained with BODIPY-DIF-3G, BODIPY-DIF-3R, or Hoechst (e) and those stained with BODIPY-DIF-3G, BODIPY-DIF-3R, or MitoTrackerDR (f) with the use of pseudo colors. Scale bars: 20 µm.