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. 2014 Mar 27;3(4):297–306. doi: 10.1242/bio.20146866

Fig. 2. Ligand-induced internalization of GFP-tagged endocytosis-defective receptors depends on presence of full-length binding-defective receptors Ste2-F204S or full-length wild-type receptors Ste2.

Fig. 2.

Cultures were treated with cycloheximide and α-factor for 15 minutes at 30°C. GFP fluorescence images and Nomarski images (DIC) are indicated below each column; first two columns are cultures lacking α-factor; the last two columns are cultures treated with α-factor. First row, cells expressed both untagged Ste2-F204S and Ste2-F204S,T326-GFP (strain DJ485-1 containing plasmid pDJ651). Second row, cells expressed both untagged Ste2 and Ste2-F204S,T326-GFP (strain DJ484-1 containing plasmid pDJ651). Third row, cells expressed both untagged Ste2 and Ste2-T326-GFP (strain DJ484-1 containing plasmid pDJ469). Bottom row, cells expressed both untagged Ste2-F204S and Ste2-T326-GFP (strain DJ485-1 containing plasmid pDJ469). Scale bar: 2 µm.