Figure 3. Analysis of the effect of P_i release at 200°.

(a,b) Histograms of the dwell time at 320° after the release from 200° arrest for 10 s, 30 s, and 60 s, or during free rotation. The bin sizes of the histogram are 15 s for a and 4 ms for b. Blue bars in a represent the number of pauses longer than 100 ms (behaviour (III)). The data after first bin in b were well-fitted by the single exponential curve where τ=9 ms (P_i release dwell at 320°). The deviations of the first bins from the single exponential curves are coloured green (behaviour (II)). (c) Probability of P_i release at 200°. The red solid line presents the simulation curve for P_i release calculated from the analysis of P_ON based on the kinetic scheme as follows: F₁·ATP↔ F₁·(ADP+P_i)→F₁·ADP+P_i. Green, blue and orange points represent the probability of occurrence of II (P_II), III (P_III) and their sum (P_II+P_III), respectively. P_II and P_III were determined from the analysis of b and a, respectively.