Table 1. Enzymatic rates of RNase splicing; the kinetic parameters of various dephosphorylated and phosphorylated states of Ire1.

	V max (μM s −1 )	K m (M)	K cat (s −1 )	Kcat/Km (M−1 s−1)	( K cat ) sample /( K cat ) dephos	( K cat / K m ) sample /( K cat / K m ) dephos
dephos Peak 1	0.024	3.68 × 10−6	0.118	3.21 × 104	1.0	1.0
dephos+ADP	0.059	6.40 × 10−6	0.295	4.61 × 104	2.5	1.4
dephos+AMP-PNP	0.029	5.48 × 10−6	0.144	2.63 × 104	1.2	0.8
dephos+ATP (in vitro re-phos)	1.36	1.62 × 10−5	6.79	4.20 × 105	57.5	13.0
Peak 2	0.28	2.78 × 10−5	1.42	5.11 × 104	12.0↓ × 5	1.6
Peak 3	1.47	2.44 × 10−5	7.37	3.02 × 105	62.4↓ × 2	9.4
Peak 4	2.5	9.95 × 10−6	12.5	1.26 × 106	105.9	39.2