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. 2014 Apr 1;5:3550. doi: 10.1038/ncomms4550

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(a) Colocalization of DRP1 foci at mitochondria by immunofluorescence of DRP1 and Tom20 in cortical neurons using confocal imaging. Arrows show DRP1 foci localized at mitochondria. Scale=5 μm. (b) Quantification of the number of DRP1 foci colocalized to mitochondria and represented as mean and s.d. (n=3). (c) Western blot of the indicated proteins from whole cell lysates of cultured cortical neurons following 6-h incubation at the indicated conditions. (d) Quantification of the number of DRP1 foci colocalized to mitochondria following 6-h hypoxic incubation in SD or AP media in the presence or absence of the fission inhibitor Mdivi-1. (e) Western blot of indicated proteins following incubation for 3 h at the indicated conditions and immunoprecipitation of endogenous (endo.) DRP1 using anti-DRP1 antibody.