Figure 5. Mild acidosis regulates cristae architecture.

(a,b) Western blot of OPA1 oligomers and monomers from lysates of BMH crosslinking in live cortical neurons at the indicated conditions. OPA1 western blot has been spliced to better clarify where monomeric and oligomerized Opa1 appear. Graphs represent the mean and s.d. (n=3) quantification of OPA1 oligomer:monomer ratio. (c–e) Representative EM images of mitochondrial ultrastructure following 6-h incubation at the indicated conditions. Scale=500 nm. Graphs in (d,e) represent mean and s.e.m (n=10) for quantification of cristae diameter and cristae number. Ctr=control represents neurons incubated in neurobasal media. (f,g) Representative images and quantification (mean and s.d., n=4) of CytC localization following CPT treatment in MES-buffered media at pH 7.2 or 6.5 in normoxia. *P<0.05; **P<0.01; ***P<0.001 (Student’s t-test).