Figure 6. Acidosis-mediated mitochondrial remodelling protects neurons from cell death during hypoxic stress.

(a) Quantification of cell death of cortical neurons as mean and s.d. (n values indicate independent experiments) following 30-h incubation in SD or AP media at the indicated conditions. (b) Mean and s.d. (n=3) of neuronal cell death analysis following 24-h treatment with CPT in MES-buffered media at pH 7.2 or 6.5 in normoxia. (c) Quantification of cell death in neurons infected with lentivirus encoding a scrambled (shCTR) or OPA1-specific (shOPA1) shRNA following 30 h in SD or AP media at the indicated conditions (mean and s.d., n=3). (d) Mean and s.d. (n=4) of cell death quantification in WT (wild-type) or MFN1-deficient (MFN1−/−) cortical neurons following 30-h incubation in SD or AP media at the indicated conditions. (e) Quantification of cell death in neurons infected with adenovirus-encoding GFP or DRP1-YFP following 30-h incubation in SD or AP media at the indicated conditions (mean and s.d., n=3). *P<0.05; **P<0.01; ***P<0.001 (Student’s t-test).