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. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591

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(a) Immunoblotting analysis of CD147 or CD9 against purified EVs isolated from CCD-18Co cells, HCT116 cells, HCT15 cells, HT29 cells, WiDr cells, COLO201 cells, COLO205 cells and SW1116 cells. EV proteins (250 ng) was used for the detection of CD147 and CD9. (b) Correlation between ExoScreen detection of CD147/CD9 double-positive EVs and EV protein concentration in a dilution series. EVs protein concentration was measured via the Qubit system. EVs were purified from CCD-18Co, HCT116, HCT15, HT29, WiDr, COLO201, COLO205 or SW1116 CM. Error bars are s.e.m. (n=3 for each condition). Data are representative of at least three independent experiments each.