Figure 5. Effect of phosphomycin, penicillin and MP265 on the localization of RodZ and MreB.

(a) Localization of RodZ and MreB in presence of different drugs. Vero cells infected with W. chondrophila were treated with the indicated drugs 2 h p.i. (500 μg ml⁻¹ phosphomycin, 500 μg ml⁻¹ penicillin or 100 μg ml⁻¹ MP265). Cells were fixed 24 h p.i., labelled with antibodies against RodZ (green) and MreB (red) and observed by confocal microscopy. Arrows show the accumulation of RodZ at mid-cell. (b) Quantification of the accumulation of RodZ and MreB at the bacterial mid-cell by confocal microscopy after treatment with 500 μg ml⁻¹ phosphomycin (Phospho), 500 μg ml⁻¹ penicillin (Peni), 500 μg ml⁻¹ piperacillin (Pip), 500 μg ml⁻¹ mecillinam (Mec) and 100 μg ml⁻¹ MP265 (n=100). (Error bars represent the s.d. of three independent experiments).