Table 1. Results and statistical analyses of C. elegans life span assays.
| Strain, substance and solvent | Maximum life span (d) (± s.d.)* | Mean life span (d) (± s.d.) | P-value (versus control, see footnotes) | Number of experiments (n) | Number of nematodes (n) |
|---|---|---|---|---|---|
| N2 H2O (1) | 25.00±0.0 | 22.01 | 1 | 135 | |
| N2 100 μM GlcN (1) | 27.00±0.0 | 23.82 | <0.0001‡ | 1 | 138 |
| N2 H2O (2) | 25.00±0.0 | 21.59 | 1 | 135 | |
| N2 100 μM GlcN (2) | 27.00±0.0 | 23.67 | <0.0001‡ | 1 | 137 |
| N2 H2O (3) | 25.00±0.0 | 21.52 | 1 | 150 | |
| N2 100 μM GlcN (3) | 27.00±0.0 | 23.14 | <0.0001‡ | 1 | 124 |
| N2 H2O (blinded) | 24.00±0.0 | 20.98 | 1 | 107 | |
| N2 100 μM GlcN (blinded) | 26.00±0.0 | 22.72 | <0.0005‡ | 1 | 126 |
| N2 H2O (HIT† bacteria) | 28.00 | 25.52 | 1 | 131 | |
| N2 100 μM GlcN (HIT† bacteria) | 32.00 | 27.99 | <0.0001§ | 1 | 138 |
| N2 H2O | 25.00±0.0 | 21.71±0.3 | 3 | 420 | |
| N2 10 μM GlcN | 25.00±0.0 | 22.20±0.3 | <0.01‡ | 3 | 428 |
| N2 100 μM GlcN | 27.00±0.0 | 23.54±0.3 | <0.0001‡ | 3 | 399 |
| N2 1 mM GlcN | 26.33±1.2 | 23.33±0.3 | <0.0001‡ | 3 | 442 |
| N2 H2O | 25.00±0.0 | 21.50±0.6 | 3 | 357 | |
| N2 100 μM GlcN | 27.00±0.0 | 23.53±0.4 | <0.0001‡ | 3 | 408 |
| N2 1 mM GlcN | 27.33±0.6 | 23.82±0.5 | <0.0001‡ | 3 | 286 |
| N2 10 mM GlcN | 27.00±0.0 | 24.05±0.2 | <0.0001‡ | 3 | 286 |
| aak-2 (ok524) H2O | 19.67±1.2 | 17.63±0.3 | 3 | 397 | |
| aak-2 (ok524) GlcN | 19.67±1.2 | 17.60±0.3 | NS|| | 3 | 397 |
| sir-2.1 (ok434) H2O | 22.67±0.6 | 19.50±0.4 | 3 | 294 | |
| sir-2.1 (ok434) GlcN | 23.00±0.0 | 20.14±0.4 | <0.05¶ | 3 | 315 |
| N2 DMSO | 23.67±1.15 | 21.06±0.42 | 3 | 348 | |
| N2 GlcN | 25.00±0.0 | 22.53±0.8 | <0.0001# | 3 | 305 |
| N2 BHA | 23.00±0.0 | 20.96±0.29 | NS# | 3 | 320 |
| N2 GlcN/BHA | 23.00±0.0 | 20.67±0.16 | NS# | 3 | 325 |
| N2 H2O | 24.33±1.2 | 21.38±0.3 | 3 | 370 | |
| N2 GlcN | 25.33±0.6 | 22.42±0.4 | <0.0005‡ | 3 | 338 |
| N2 NAC | 24.67±1.5 | 21.73±0.4 | NS‡ | 3 | 372 |
| N2 GlcN/NAC | 24.33±1.2 | 21.05±0.4 | NS‡ | 3 | 346 |
| pmk-1 (km25) H2O | 24±0.0 | 20.46±0.3 | 6 | 456 | |
| pmk-1 (km25) GlcN | 24±0.0 | 20.76±0.3 | NS** | 6 | 388 |
| daf-16 (mu86) H2O | 21.00±0.0 | 18.27±0.3 | 3 | 443 | |
| daf-16 (mu86) GlcN | 21.00±0.0 | 19.01±0.3 | <0.05†† | 3 | 431 |
| skn-1 (zu135) H2O | 19.67±1.2 | 17.34±0.5 | 3 | 200 | |
| skn-1 (zu135) GlcN | 19.00±0.0 | 16.64±0.5 | NS‡‡ | 3 | 190 |
| F21D5.1 RNAi/H2O | 30.0 | 24.46 | 1 | 184 | |
| F21D5.1 RNAi/100 μM GlcN | 35.0 | 28.24 | <0.0001§§ | 1 | 178 |
| aat-1 RNAi/H2O | 20.00±0.4 | 21.78±0.3 | 3 | 214 | |
| aat-1 RNAi/100 μM GlcN | 20.00±0.4 | 21.59±0.3 | NS|||| | 3 | 225 |
NS, not significant.*75th percentile; †HIT, heat-inactivated;
‡Controls: N2 H2O; §N2 H2O HIT bacteria; ||aak-2 (ok524) H2O; ¶sir-2.1 (ok524) H2O; #N2 DMSO; **pmk-1 (km25) H2O; ††daf-16 (mu86) H2O; ‡‡skn-1(zu135) H2O; §§F21D5.1 RNAi/H2O; ||||aat-1 RNAi/H2O.