Figure 5.

Loss of BAF180 Leads to Hypersensitivity to DNA Damage, Increased Frequency of Chromosome Aberrations, and Dynamic CIN

(A) Viability curves of WT (+/+) or BAF180 knockout (−/−) mESCs (left panel), and control and BAF180-depleted 1BR-hTERT cells (right panel) following exposure to MMC.

(B) The presence of chromosomal aberrations in metaphase spreads prepared from WT (+/+) or BAF180 knockout (−/−) mESCs following exposure to MMC was analyzed and presented as frequency per 100 chromosomes. Images representative of each category are shown in the top panels.

(C) Quantification of micronuclei present in WT (+/+) or BAF180 knockout (−/−) mESCs following exposure to MMC.

(D) Distribution curves showing chromosome numbers from WT (+/+) and BAF180 knockout (−/−) mESCs following exposure to MMC.

See also Figure S5.