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Calibration of the Number of Base Pairs Unwound to FRET
(A) DNA construct schematics.
(B) Representative calculated FRET efficiency versus time traces for 10 AT and 30 GC substrates (right and left panels).
(C) FRET histogram. The arrows indicate the region of the FRET values used to build the histogram; >50 molecules were used to build the histogram. The histogram peaks at 0.3 FRET.
