Figure 1.

Early RET AutoP Sites Map to Regions outside the KD Core

(A) Schematic diagram of discrete RET functional domains together with the autoP sites analyzed in this study.

(B) Global temporal autoP analysis by LFQMS of recombinant purified RET ICD (2.5 μM) treated with saturating concentrations of ATP (5 mM) and MgCl₂ (10 mM) for 0–120 min. Data represent the mean signal value (Log₂ ratios of phosphorylated peptides standardized to the nonphosphorylated counterparts) ± SE of four independent experiments (n = 4) performed with different protein preparations.

(C) WB analyses of samples analyzed in (B) using the indicated antibodies.

(D) Phosphorylation status (saturation rate, percentage) for each LFQMS-identified autoP site and phosphorylation state (1P to 6P) from (B).

(E) Cumulative phosphorylation for each autoP site from (D).

See also Figure S1.