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. 2014 Mar 6;53(5):738–751. doi: 10.1016/j.molcel.2014.01.015

Figure 3.

Figure 3

Oncogenic Mutations in the RET Tyrosine KD Perturb the AutoP Trajectory

(A) WB analysis of RET ICD (2.5 μM) WT and oncogenic mutants V804M and M918T stimulated with ATP (5 mM) and MgCl2 (10 mM) for 0–120 min using the indicated antibodies.

(B) Quantitation by densitometry analysis of experiments depicted in (A). Data represent the mean signal value (percentage) ± SE, n = 6. For clarity, only Y905 and Y1015 are depicted.

(C) Temporal autoP analysis by LFQMS of recombinant purified RET ICD (2.5 μM) WT, V804M, or M918T treated with ATP (5 mM) and MgCl2 (10 mM) for 0–10 min. Data represent the mean signal value (Log2 ratios of phosphorylated peptides standardized to the nonphosphorylated counterparts) ± SE, n = 2.