Fig. 3.

Assessment of size variations in TatAd complexes. (A) Initial centred sum image of 1990 particles. (B) Size class averages of TatAd reveal a slight size variation that correlates with internal density (see largest class, top right). The numbers of particles per class are as follows: Class 1, 547; Class 2, 477; Class 3, 440; Class 4, 352. (C) Size classes after reference-free rotational and translational alignment of the untilted particles. (D) Reprojections of TatAd 3D density maps for comparison to the class averages. Scale bar = 10 nm.