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. 2013 Aug;1833(8):1811–1819. doi: 10.1016/j.bbamcr.2013.03.028

Table 1.

Plasmids and strains used in this work.

Plasmids/cells Relevant properties Reference/source
pBAd-his pBAD24 derivative containing the B. subtilis tatAd-his gene; Ampr [21]
MC4100 FΔlacU169 araD139 rpsL150 relA ptsF rbs flbB5301 [36]
MC4100 ΔtatABCDE tat deletion strain [8]
MC4100 ΔtatAE tatA and tatE deletion strain [8]
MC4100 ΔtatB tatB deletion strain [8]
MC4100 ΔtatC tatC deletion strain [8]
pBAD-A-Strep pBAD24 derivative containing the E. coli tatA gene with a C-terminal Strep-II™ tag; Ampr [37]
pBAD-Ac-Strep pBAD24 derivative containing the B. subtilis tatAc-strepII gene; Ampr This study

Ampr = ampicillin resistant.